The luxury Perthshire resort, due to open a new, large-scale conference centre in the spring, has objected to the relocation of this year's T in the Park festival, which is moving from Balado, 12.4 miles away to Strathallan, just 1.8 miles from the hotel.
Agents Colliers International, working on behalf of Gleneagles, cited "real concern" about noise levels from the event's main stage, which faces towards the hotel, adding that it "cannot support the application as it currently stands" and that it was worried guests could be "subject to anti-social behaviour". It also highlighted the "strong likelihood" of unauthorised satellite campsites being set up in the surrounding area.
Gleneagles managing director Bernard Murphy said the hotel had not formally objected to the event taking place, but had raised concerns over "noise, traffic management and public nuisance".
He said: "We hope that by raising these matters at an early stage, DF Concerts and the various public agencies involved will be able to be address them to minimise their impact on the local community.
"We recognise the appetite for this event and how important it is for tourism, but also want to ensure the best possible experience for our guests."
The comment on the application was lodged on the same day the Scottish Environment Protection Agency withdrew its opposition to the plans.
T in the Park representatives said they were in discussions with the hotel on the matter.
Gleneagles, which hosted the Ryder Cup last September, is a C&I favourite and has been put up for sale this year by owners Diageo and it valued at around £200m.