The mixed-use development is a new landmark in the city which also includes a convention centre, commercial outlets, academic and entertainment facilities, office buildings and residences.
The hotel has 215 guest rooms and four meeting and event spaces, all featuring views of the water and greenery surrounding the property.
There is also a gym, spa, an indoor pool, two restaurants and a bar.
Hafidh Al Busaidy, general manager of the Intercontinental Osaka, said: "We aim to provide contemporary luxury to our guests in what is the city’s most exciting new development.
"Our team offers world-class service to both international and local guests alike and we hope to provide them with new and unexpected experiences when they stay, dine or hold an event with us."