A new study has shown that the Sheffield International Venues (SIV) facility attracted more than 125,000 visitors from outside the city to the stadium in the 12 months to May 2012
Around 200 commercial events were hosted at the stadium, with Adidas and McDonald's among the corporates hiring the space.
A Royal College of Psychiatry event, the National Bonsai exhibition, property auctions, and team-building days are among the highlights of the past year.
Don Valley also staged the biggest Sheffield Half Marathon on record with nearly 6,000 runners starting and finishing the race at the stadium.
Pat Smith, general manager of Don Valley, said: "It has been another great year. Key to this success has been the adaptability and flexibility of the venue. From national exams to concerts and film shoots to team building, every day is busy with different activities."
Don Valley will host ‘Sheftival’ on 4 and 5 August to celebrate London 2012. It is being billed as the city’s biggest-ever festival of sport and culture and is expected to attract more than 50,000 people to the city's Olympics party.