The 218-bedroom hotel, owned and operated by Peel Leisure, opened in the £650m Media City UK development in November 2010 and features a gym, three meeting rooms and two restaurants.
The Holiday Inn Manchester Media City UK joins the Ramada Manchester Salford Quays and The Old Trafford Lodge in the Quays Partnership.
Other partners currently include Imperial War Museum North, Lancashire County Cricket Club, Lowry Outlet Mall, The Lowry, Media City UK and Mersey Ferries.
The initiative works in partnership with Marketing Manchester.
Peel Leisure director Peter de la Perrelle said: "The Quays Partnership allows us to co-ordinate all our individual activities to the mutual benefit of all partners and is a key factor in bringing this wonderful location to life."