The Manchester-based agency was appointed after organising the trust's inaugural event in 2009.
The 2010 conference will be held at the MK Don Stadium on 3 June and delegates will include executive staff from every club in the Football League, plus delegates from stakeholder groups including the Professional Footballers Association (PFA), academia and the media.
The event will include keynote addresses from the PFA chairman Gordon Taylor, and NW Strategic Health Authority chief executive Mike Farrar.
HGA Creative's remit includes providing online registration, a supplier exhibition with more than 30 different stands, technical staging and production.
Football League Trust general manager Dave Edmundson said: "HGA's ability to put together a creatively engaging conference is remarkable. Through this period we have built up a strong relationship - one built on trust and understanding."