The gardens tourist attraction has submitted plans to Cornwall Council for a new 115-bedroom mid-range hotel at Bodelva, near St Austell.
Its executive director David Harland said the accommodation would "secure Eden’s identity as a conferencing and events venue".
Since opening in 2001, Eden has attracted 16m visitors who have spent at least £1.6bn in Cornwall, according to its research. The hotel, it argues, would help sustain business all year round and unlock the potential of the wider estate beyond its famous biomes.
If the planning application is successful, the new timber-clad development, which would be funded by the private sector, would create around 25 new jobs.
Mr Harland said: "Establishing accommodation on-site has been an aspiration for Eden since it began and we are now in a strong position to make this a reality.
"The proposed hotel is intended to support Eden's activities, its educational aspirations, its existing and future conference events, weddings, and the annual Eden Sessions series of concerts.
"Many of these events require visitors to arrive the previous evening and then remain on site until the next day or day after. This new accommodation would enable us to meet their expectations and secure Eden's identity as a conferencing and events venue."
The hotel would also enable the Building Research Establishment's National Solar Centre and Cornwall Sustainable Building Trust's Green Build Hub to offer residential courses to support their own training ambitions.
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